Small non-coding RNAs (smRNAs) regulate gene expression via diverse mechanisms and facilitate fundamental cellular processes such as transcript splicing and protein translation. Consistent performance across a range of input amounts and sample types:Ĭomparable sequencing results are obtained for 1 ng–2 µg inputs of total RNA or enriched small RNA.In addition to miRNAs, this approach allows for capture of piRNAs, snoRNAs, snRNAs, etc. RNA 3′ polyadenylation and SMART template-switching technology capture small RNAs with greater accuracy than approaches involving adapter ligation. Small RNA library preparation with reduced bias:.Some of those genes have human orthologue with potential therapeutic targets that will help to better understand pathologies related to the invalidation of those pathways. Our team's projects aim to better understand the dynamics (activation and evolution) of piRNA clusters in heterochromatic locus from which piRNAs are maturated and, using functional screens, to characterize genes involved in siRNA- and piRNA-dependent repression. In Drosophila, their expression are controlled by piRNAs in the gonads and by siRNAs in somatic tissues. TEs that are present in all genomes, have the ability to transpose thus causing possibly deleterious mutations. In metazoans, three types of small RNAs have been described, whose specificity differs according to the Argonaute protein with which they interact: microRNA (miRNA), small interfering RNA (siRNA) and PIWI interacting RNAs (piRNA). We are also interested in RNAs post-transcriptional modifications that play a fundamental role in RNA biogenesis and fate. Our team studies the Biology of RNAs, in particular the small RNAs involved in the regulation of gene expression and Transposable Elements (TE). Stochastic dynamics of reactive and live systems.Biomembrane plasticity and cellular function.MEchanics of integrated and artificial Biological Systems.Dynamic and multiscale processes of auto-organisation in tissue morphogenesis.Cortical Actomyosin Dynamics in Development and Morphogenesis (CADMO).Compartmentation and intracellular traffic of mRNPs.Induction and Differentiation during Vertebrate Embryonic Development.TErBio: Transgenerational Epigenetics & small RNA biology.Epigenetic Control of Developmental Homeostasis and Plasticity.Migration and Differentiation of Hematopoietic Stem Cells.Formation and Interaction of Neural Networks (FINN).Development and Plasticity of Neural Network.Cerebellum, Navigation and Memory (CeZaMe).Neuroplasticity of Reproductive Behaviors.Development of the Spinal Cord Organization.Neuronal Networks and Physiopathological Rhythms.Epigenetics and RNA metabolism in human diseases. ![]() Stem Cells, Cardiovascular pathophysiology and Biotherapies (CARTHER).Dynamics of Intracellular Signaling and Therapeutic Targets.Compensation systems in neurodegenerative disease and aging (Brain-C).Integrative Cellular Ageing and Inflammation (ICAI).FIONA - Functional Imaging Of Nuclear Architecture.Synthetic and Systems Biology of Microalgae.Statistical Genomics and Biological Physics.Computational and Quantitative Biology + ×
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